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Immunoprecipitation and immunoblotting analyses

YJ Yoshikazu Johmura
MN Makoto Nakanishi

Last updated date: Aug 20, 2021 Views: 694 Forks: 0

original An abbreviated version of this protocol was published in Science
Senolysis by glutaminolysis inhibition ameliorates various age-associated disorders
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Thank your the questions.

We attached the file containing the full blots (KGA, GAC, and GLS1) with molecular markers and the antibody information in Figure 1D. 

 

Sincerely,

Yoshikazu Johmura

 

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temp Fgiures for reguest from a reader of Science article.pptx download
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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Johmura, Y and Nakanishi, M(2021). Immunoprecipitation and immunoblotting analyses. Bio-protocol Preprint. bio-protocol.org/prep1346.
  2. Johmura, Y., Yamanaka, T., Omori, S., Wang, T., Sugiura, Y., Matsumoto, M., Suzuki, N., Kumamoto, S., Yamaguchi, K., Hatakeyama, S., Takami, T., Yamaguchi, R., Shimizu, E., Ikeda, K., Okahashi, N., Mikawa, R., Suematsu, M., Arita, M., Sugimoto, M., Nakayama, K. I., Furukawa, Y., Imoto, S. and Nakanishi, M.(2021). Senolysis by glutaminolysis inhibition ameliorates various age-associated disorders. Science 371(6526). DOI: 10.1126/science.abb5916

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