Construction of POC diagnostic device

Setting up the device:

1 -3D print the desired miSHERLOCK device and components (heater platform, device housing, saliva collector, plunger, and sample preparation column) using black resin. Files for the duplex, triplex, and quadruplex devices can be found here. 

2 - Assemble the temperature control, LED, and temperature circuits following the circuit diagram provided, which  can be found in Fig. S15 here. 

3 - Attach heaters to the heater platform and insert the LEDs and temperature control circuit into the miSHERLOCK housing. Images of how to correctly insert them can be found in Fig. S16 here.

4 - Cut the 2mm orange acrylic for the transilluminator ( 2.75 cm × 2.25 cm, 2.75 cm × 3.2 cm, or 2.75 cm × 4.0 cm for the duplex, triplex, or quadruplex transilluminator filter, respectively)  

4 - Attach the 4mm PES filter to the bottom of the sample preparation column.

5 - Insert Whatman gel blotting paper to the correct region on the heater platform.

6 - Line the inside of the reservoir on the miSHERLOCK device housing  with double-sided tape and attach foil to the underside of the reservoir, making sure that it is fully sealed.

7 - Fill each reservoir with 50uL nuclease-free water.

8 - insert new SHERLOCK assays to the bottom of the chamber.

9 - Connect heaters to the battery power when ready to use.