Antibiotic treatment

Mice were orally gavaged daily for five days with either 0.25 ml autoclaved deionized water or 0.25 ml of an antibiotic cocktail, each administered with a sterile oral gavage needle, one per mouse. The antibiotic cocktail consisted of ampicillin 1 mg/ml, vancomycin 5 mg/ml, neomycin 10 mg/ ml, and metronidazole 10 mg/ml made up in autoclaved deionized water in excess of daily needs. Fresh antibiotic cocktails were prepared every day. For more information on the antibiotic cocktail protocol see Reikvam et al. 2011 PLoS One. 

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