The main steps are carried out according to the instructions. Would you tell me your specific questions?
The instruction is attached.
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How to cite:Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
- Zhang, S(2021). 2.8. Cellular senescence quantitative assay. Bio-protocol Preprint. bio-protocol.org/prep1278.
- Lin, X., Dai, Y., Tong, X., Xu, W., Huang, Q., Jin, X., Li, C., Zhou, F., Zhou, H., Lin, X., Huang, D. and Zhang, S.(2020). Excessive oxidative stress in cumulus granulosa cells induced cell senescence contributes to endometriosis-associated infertility. Redox Biology 30. DOI: 10.1016/j.redox.2020.101431
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