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Lentiviral constructs and library construction

DS Daniel Schramek

Last updated date: Jun 13, 2021 Views: 692 Forks: 0

original An abbreviated version of this protocol was published in Science in Mar, 2020
Rare driver mutations in head and neck squamous cell carcinomas converge on NOTCH signaling
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We have published a very detailed protocol regarding Lentiviral constructs and library construction in the following methods paper:

Vis Exp. 2020 Nov 2;(165).

doi: 10.3791/61693.

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Sampath Kumar Loganathan 1, Ahmad Malik 2, Ellen Langille 2, Chen Luxenburg 3, Daniel Schramek 4

Copyright: Content may be subjected to copyright.
How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Schramek, D(2021). Lentiviral constructs and library construction. Bio-protocol Preprint. bio-protocol.org/prep1158.
  2. Loganathan, S. K., Schleicher, K., Malik, A., Quevedo, R., Langille, E., Teng, K., Oh, R. H., Rathod, B., Tsai, R., Samavarchi-Tehrani, P., Pugh, T. J., Gingras, A. and Schramek, D.(2020). Rare driver mutations in head and neck squamous cell carcinomas converge on NOTCH signaling . Science 367(6483). DOI: 10.1126/science.aax0902

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