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Preprint

Cuticle preparations

YY Yoseop Yoon
US Urs Schmidt-Ott

Last updated date: Dec 11, 2019 Views: 1333 Forks: 0

original An abbreviated version of this protocol was published in eLife in Oct, 2019
Embryo polarity in moth flies and mosquitoes relies on distinct old genes with localized transcript isoforms
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Cuticles were prepped four to five days after injection. Eggshells was removed with tungsten needles and the embryos were transferred to a glass block dish with a drop of 1:4 glycerol/acetic acid. Following incubation in 1:4 glycerol/acetic acid overnight at room temperature, the cuticles were transferred onto a glass slide, oriented, mounted in 1:1 Hoyer’s medium/lactic acid (Stern and Sucena, 2000), covered with a cover glass, and dried overnight at 65 °C.

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Yoon, Y and Schmidt-Ott, U(2019). Cuticle preparations. Bio-protocol Preprint. bio-protocol.org/prep113.
  2. Yoon, Y., Klomp, J., Martin-Martin, I., Criscione, F., Calvo, E., Ribeiro, J. and Schmidt-Ott, U.(2019). Embryo polarity in moth flies and mosquitoes relies on distinct old genes with localized transcript isoforms. eLife. DOI: 10.7554/eLife.46711

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