Open-field test

Open-field test 

1. This take was used a black square box (60 × 60 × 30 cm3) including a center (30 × 30 cm2).

Note: Behavior was measured in a quiet and low-light intensity (<50 lx) room. 

2. Rat was placed in a black square box for 5 min to habituation.

Note: When placing the rat in the box, place it in the center to begin exploring (staring point=center zone).

3. After habituation, the measurement has started the box for 15 min.

4. Behavior was video-recorded for later analysis using a SMART v3.0 video tracking system (Panlab, S.L.U., Barcelona, Spain). 

5. We analyzed the total distance (cm), velocity (cm/s), and resting times in the center zone (s).

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