Published: Vol 3, Iss 21, Nov 5, 2013 DOI: 10.21769/BioProtoc.953 Views: 10246
Reviewed by: Anonymous reviewer(s)
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Abstract
Stationary phase bacteria are highly tolerant to hydrogen peroxide. This protocol was developed to test the susceptibility to hydrogen peroxide killing in different Pseudomonas aeruginosa strains. This assay provides a reliable way to measure killing of stationary phase bacterial cells to hydrogen peroxide and can be adapted to test other oxidants.
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
We would like to acknowledge CIHR (MOP-102727 to DN) and the Burroughs Wellcome Fund (1006827.01 to DN) for funding. This protocol was adapted from the previously published paper Khakimova et al. (2013).
References
Article Information
Copyright
© 2013 The Authors; exclusive licensee Bio-protocol LLC.
How to cite
Khakimova, M. and Nguyen, D. (2013). H2O2 Kill Assays of Planktonic Stationary Phase Bacteria. Bio-protocol 3(21): e953. DOI: 10.21769/BioProtoc.953.
Category
Microbiology > Antimicrobial assay > Killing assay
Biochemistry > Other compound > Reactive oxygen species
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