Update Notice: Immunopeptidomics Workflow for Isolation and LC-MS/MS Analysis of MHC Class I-Bound Peptides Under Hypoxic Conditions

After official publication of our protocol in Bio-protocol (https://bio-protocol.org/e5505), it has been brought to our attention that the legend for Figure 2 requires a citation to indicate that the protocol is adapted from a previous report. We would also like to take this opportunity to update the acknowledgements section of the paper.

Updated Figure 2 legend:
Figure 2. Generation of MHC immunoaffinity column. The first six steps of the procedure are shown and as previously described [13]. CV, column volume; DMP, dimethyl pimelimidate dihydrochloride.

Updated Acknowledgements:
Acknowledgments
H.E.: Conceptualization, Investigation, Writing-Original draft, Writing-Review & Editing. E.M.H.: Funding acquisition, Supervision, Writing-Review & Editing. E.A.: Writing-Review & Editing. This work was supported by Medical Research Council‐UKRI grant MC_UU_00001/8, Cancer Research UK RadNet Centre Award C6078/A28736, NIHR Oxford BRC, NIHR203311. This protocol was used in [28]. The protocol was modified and adapted from [13]. We are grateful to Drs Tailor and Parker and Prof. Ternette (University of Oxford) for their expert advice in the development of this protocol. The following figures were created using BioRender: Graphical overview, BioRender.com/BM28VRHAEH; Figure 2, BioRender.com/CN28VRHTLV; Figure 3, BioRender.com/AY28VRI5PW.

This article was previously updated on Feb 20, 2026. The update notice can be found here: https://bio-protocol.org/e5630