Germinal center is the primary site for B cells to undergo somatic hypermutation and class switching. A recently discovered subset of T cells known as T follicular helper (TFH) cells are essential for germinal center reaction and thus have been the subjects of intensive study in the recent years. This protocol describes a reliable way to stain this population for flow analysis. This protocol was developed or modified in Dr. Anne Davidson’s lab at Feinstein Institute for Medical Research.

This protocol describes how to separate the endoplasmic reticulum (ER) and Golgi apparatus in yeast cells using a subcellular fractionation approach with an Accudenz gradient.