Abstract
Peritoneal macrophages are used as primary macrophages in lots of studies, mainly because they are easy to obtain. Injection of thioglycollate broth i.p. induces inflammatory responses and elicits large numbers of macrophages. This protocol can be used for harvesting resident or thioglycollate-elicited peritoneal cells. Peritoneal macrophages are non-adherent in situ and when they are cultured in dishes, they become adherent so that macrophages may be separated from other types of cells in peritoneal cavity.
Materials and Reagents
Equipment
Procedure
Note: The study was performed under an IACUC approved protocol (LCID 11E).
Recipes
Acknowledgments
This research was supported by the Intramural Research Program of the NIH, NIAID
References
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