Abstract
Depending on the species, embryo sacs can be difficult to observe. Ovule clearing allows precise observation of whole tissues under Differential interference contrast (DIC) microscopy. The use of Methyl Salicylate as a clearing agent has proved to be particularly reliable for Solanaceous species.
Keywords: Solanum, Ovule, Reproduction, Embryo sac, Differential interference contrast (DIC)
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol is adapted from Chevalier et al. (2013) and Estrada-Luna et al. (2004).
References
If you have any questions/comments about this protocol, you are highly recommended to post here. We will invite the authors of this protocol as well as some of its users to address your questions/comments. To make it easier for them to help you, you are encouraged to post your data including images for the troubleshooting.