Abstract
Chronic inflammation drives initiation of hepatocellular carcinoma (HCC), but the underlying mechanisms linking inflammation and tumor formation remain obscure. In this study, soft agar anchorage-independent assay were used to determine tumor transform activity of hepatoma cells with ISX over expression or knockdown in vitro.
Materials and Reagents
Equipment
Procedure
Acknowledgments
This protocol was adapted from Hsu et al. (2013).
References
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