Published: Vol 3, Iss 20, Oct 20, 2013 DOI: 10.21769/BioProtoc.933 Views: 14828
Reviewed by: Anonymous reviewer(s)
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Abstract
To get activated, T cells need to find their cognate antigen at the surface of an antigen-presenting cell (APC). Recognition of cognate antigen in the context of the MHC (Major histocompatibility complex) by the TCR (T-Cell Receptor) results in long lasting interactions between T cells and APCs. Subsequently, T cells form homotypic interactions with each other, which is seen as a hallmark of T cell activation. This protocol describes a method to analyze T-APC and T-T conjugation.
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Acknowledgments
This protocol was adapted from the previously published paper Gerard et al. (2013). This protocol was adapted in the laboratory of Matthew F. Krummel, and was supported by grants from the Juvenile Diabetes Foundation (MFK), and NIH R01AI52116 (MFK).
References
Article Information
Copyright
© 2013 The Authors; exclusive licensee Bio-protocol LLC.
How to cite
Gérard, A. (2013). In vitro T Cell–DC and T Cell–T Cell Clustering Assays. Bio-protocol 3(20): e933. DOI: 10.21769/BioProtoc.933.
Category
Immunology > Immune cell function > Lymphocyte
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