Abstract
To get activated, T cells need to find their cognate antigen at the surface of an antigen-presenting cell (APC). Recognition of cognate antigen in the context of the MHC (Major histocompatibility complex) by the TCR (T-Cell Receptor) results in long lasting interactions between T cells and APCs. Subsequently, T cells form homotypic interactions with each other, which is seen as a hallmark of T cell activation. This protocol describes a method to analyze T-APC and T-T conjugation.
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol was adapted from the previously published paper Gerard et al. (2013). This protocol was adapted in the laboratory of Matthew F. Krummel, and was supported by grants from the Juvenile Diabetes Foundation (MFK), and NIH R01AI52116 (MFK).
References
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