Published: Vol 3, Iss 19, Oct 5, 2013 DOI: 10.21769/BioProtoc.930 Views: 14167
Reviewed by: Lin FangFanglian HeAnonymous reviewer(s)
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Abstract
Normal pancreatic acinar cells are difficult to maintain on traditional plastic culture surfaces due to their physical properties of housing large quantities of digestive enzymes and the formation of intercellular tight junctions and gap junctions (Apte and Wilson 2005; Rukstalis et al., 2003). However, placing primary acinar cells within a 3-dimensional matrix (3D-culture) maintains the cells for sufficient time so that they can be monitored for physiological changes to different stimuli. We have used a modified collagen 3D-culture system that has been adapted from Means et al. (2005) to model the very early events associated with pancreatic cancer development. In this model, KrasG12D-expressing pancreatic acinar cells, or wildtype acinar cells treated with EGFR-dependent growth factors (i.e., TGFα), convert to ductal cysts that mimic the acinar-to-ductal metaplasia (ADM) stage that precedes formation of Pancreatic Intraepithelial Neoplasia (PanIN) and Pancreatic Ductal Adenocarcinoma (PDAC) (Means et al., 2005; Shi et al., 2013).
Keywords: Tissue cultureMaterials and Reagents
Equipment
Procedure
The following procedures for one mouse pancreas
Notes:
Recipes
Acknowledgments
This protocol was adapted from Shi et al. (2013).
References
Article Information
Copyright
© 2013 The Authors; exclusive licensee Bio-protocol LLC.
How to cite
Qu, C. and Konieczny, S. F. (2013). Pancreatic Acinar Cell 3-Dimensional Culture. Bio-protocol 3(19): e930. DOI: 10.21769/BioProtoc.930.
Category
Cancer Biology > General technique > Cell biology assays
Cell Biology > Cell isolation and culture > 3D cell culture
Cell Biology > Tissue analysis > Tissue isolation
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