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Batch Culture Fermentation of Endophytic Fungi and Extraction of Their Metabolites   

Edited by
Ru Zhang
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Original research article

A brief version of this protocol appeared in:
PLOS ONE
Feb 2013

Abstract

Antibiosis is one of the possible modes of action shown by endophytic fungi having antifungal activity. To test if antifungal activity in endophytic fungi is due to antibiosis, assay of the metabolites of endophytic fungi was needed. To obtain metabolites for bioassay batch culture fermentation and extraction of metabolites was done. Fungus was multiplied on wickerham media at incubation temperature of 25 ± 2 °C for 4 weeks and then extracted with solvents of different polarity. All the solvent extracts were dried under vacuum rotary evaporator to get dried crude fungal extract, which was subjected to further fractionation and bioassay.

Keywords: Fermentation, Endophytic fungi, Batch culture, Metabolites of endophytic fungi, Solvent extraction

Copyright: © 2013 The Authors; exclusive licensee Bio-protocol LLC.
How to cite: Kumar, S. and Kaushik, N. (2013). Batch Culture Fermentation of Endophytic Fungi and Extraction of Their Metabolites. Bio-protocol 3(19): e926. DOI: 10.21769/BioProtoc.926.
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