Abstract
Spindle angles measures derive from the measures of spindle poles positions that were taken from fixed and immunostained adherent cells. To determine spindle angles (α), z-stack images of metaphasic cells immunostained with anti γ-tubulin (spindle poles) and anti β-tubulin antibodies (mitotic spindle) were acquired. A very simple ImageJ software macro was developed to measure the spindle angle using spindle pole coordinates (see Figure 1).
Keywords: Spindle, Angle, Mitosisi
Figure 1. Spindle angle measurement principle. Spindle poles coordinates are measured, then the spindle angle alpha is calculated.
Materials and Reagents
Equipment
Software
Procedure
Recipes
Acknowledgments
This protocol is adapted from: Bompard et al. (2013). GB was supported by a grant from ‘Fondation pour la Recherche Médicale’ (Université Montpellier 2). This work was supported by a grant MEGAPAK to NM from the ANR (Agence Nationale pour la Recherche) GENOPAT.
References
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