Abstract
This is a protocol to extract intact Golgi Membranes from HeLa cells using sucrose gradient centrifugation. This extraction is very useful for several applications including pull-down of Golgi membrane proteins, electron microscopy and reconstitution of protein transport into an isolated system. Protocol adapted from Balch et al. (1984).
Keywords: Golgi membranes, HeLa cells, Sucrose gradient cenrifugation
Materials and Reagents
Equipment
Procedure
Notes
Recipes
Acknowledgments
The protocol was adapted from the original version published by Balch et al. (1984).
References
If you have any questions/comments about this protocol, you are highly recommended to post here. We will invite the authors of this protocol as well as some of its users to address your questions/comments. To make it easier for them to help you, you are encouraged to post your data including images for the troubleshooting.