Abstract
A hallmark of cancer-associated inflammation is the infiltration of leukocytes into tumors, which is believed to be recruited by chemokines. Some infiltrating leukocytes such as macrophages often promote tumor growth by producing growth-inducing and angiogenic factors. Here, we described a method of isolating tumor-infiltrating leukocytes with Percoll density gradient, because Percoll possesses a low viscosity, a low osmolarity and no toxicity to cells. Different leukocyte populations are isolated based on their density and collected at the interface between 40% and 80% discontinuous Percoll gradient.
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol is adapted from Liu et al. (2013).
References
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tumor cell also appears in the midlayer in my experiment
how about other cells?
not tested yet, I used B16F10 as a model.
I use B16 model too
Have you solve the problem?