Published: Vol 3, Iss 17, Sep 5, 2013 DOI: 10.21769/BioProtoc.892 Views: 43513
Reviewed by: Lin FangFanglian He
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Abstract
A hallmark of cancer-associated inflammation is the infiltration of leukocytes into tumors, which is believed to be recruited by chemokines. Some infiltrating leukocytes such as macrophages often promote tumor growth by producing growth-inducing and angiogenic factors. Here, we described a method of isolating tumor-infiltrating leukocytes with Percoll density gradient, because Percoll possesses a low viscosity, a low osmolarity and no toxicity to cells. Different leukocyte populations are isolated based on their density and collected at the interface between 40% and 80% discontinuous Percoll gradient.
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Acknowledgments
This protocol is adapted from Liu et al. (2013).
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Copyright
© 2013 The Authors; exclusive licensee Bio-protocol LLC.
How to cite
Liu, Y., Chen, K., Wang, C., Gong, W., Yoshimura, T., Wang, J. M. and Liu, M. (2013). Isolation of Mouse Tumor-Infiltrating Leukocytes by Percoll Gradient Centrifugation. Bio-protocol 3(17): e892. DOI: 10.21769/BioProtoc.892.
Category
Cancer Biology > General technique > Cell biology assays
Cancer Biology > Tumor immunology > Biochemical assays
Cell Biology > Cell isolation and culture > Cell isolation
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