Abstract
Particle bombardment has been shown to be a useful method to study gene promoter regulatory elements by transient transformation of maize embryos with different constructions of gene promoters fused to a gene reporter. DNA to transfer is coated to high density gold microparticles and introduced into cells when accelerated by a helium pulse. This method allows a first rapid approach, avoiding time consuming stable transformation of maize plants and also allows quantitative promoter expression analysis by a histochemical or fluorometric assay.
Keywords: Particle bombardment, Maize embryos, Promoter expression, Quantitative analysis
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol is adapted from Jose-Estanyol and Puigdomenech (2012).
References
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