Abstract
Bronchoalveolar lavage (BAL) is a simple but valuable and typically performed technique commonly used for studying the pathogenesis of lung diseases such as asthma and COPD. Cell counts can be combined with new methods for examining inflammatory responses, such as ELISA, Flow cytometric analysis, immunohistochemistry, quantitative polymerase chain reaction, and HPLC to assess cellular expression for inflammatory cytokines and growth factor. Here we describe a basic procedure to collect BAL fluid and digest lung tissue for assessing a number of pulmonary components.
Keywords: Mouse, Lung function, Pulmonary disease, Immune cell function, Mucosal immunology
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
We thank the members of the Ziegler laboratory for discussion.
References
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