Abstract
The protocol has four sub-protocols, which are about the measurement of malondialdehyde, chlorophyll proline, soluble sugar, and glutathione content, respectively, in Arabidopsis seedling by using spectrophotometer. These methods are simple, effective and reproducible, which will help the researchers who are not familiar with these approaches, quickly get reliable results.
Keywords: Malondialdehyde, Proline, Sugar, Glutathione, Arabidopsis
I. Measurement of Malondialdehyde
Materials and Reagents
Equipment
Procedure
Note: The experiment is done at room temperature (RT) except of specific indication.
II. Measurement of chlorophyll
Note: The experiment is done at room temperature.
III. Measurement of Proline
Recipes
IV. Measurement of Soluble Sugar
V. Measurement of Glutathione
Acknowledgments
This protocol is adapted from Huang et al. (2005) as well as other works mentioned in the reference list.
Competing interests
The authors declare no conflict of interest or competing interest.
References
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