Published: Vol 3, Iss 13, Jul 5, 2013 DOI: 10.21769/BioProtoc.815 Views: 10999
Reviewed by: Lin Fang
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Abstract
Mesothelial cells (MCs) form a single squamous epithelial cell layer and cover the surfaces of the internal organs, as well as the walls of cavities. The isolation of MCs is of great importance to study their function and characteristics for the understanding of physiology and pathophysiology of the liver. Glycoprotein M6a (GPM6A) was originally identified as a cell surface protein expressed in neurons and recently its expression was reported in epicardium and liver MCs (Wu et al., 2001; Bochmann et al., 2010; Li et al., 2012). Here we describe a method to isolate MCs from the adult mouse liver with anti-GPM6A antibodies. Under the low glucose and serum concentration, primary MCs grow and form epithelial colonies (Figure 1).
Figure 1. Liver MCs 2 days in culture (20x objective)
Materials and Reagents
Equipment
Procedure
I. Before procedure
II. Isolation and culture of liver mesothelial cells
Recipes
References
Article Information
Copyright
© 2013 The Authors; exclusive licensee Bio-protocol LLC.
How to cite
Li, Y., Lua, I. and Asahina, K. (2013). MACS Isolation and Culture of Mouse Liver Mesothelial Cells. Bio-protocol 3(13): e815. DOI: 10.21769/BioProtoc.815.
Category
Cell Biology > Cell isolation and culture > Cell isolation
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