Abstract
Bacterial conjugation of plasmids is the common method of introducing foreign DNA into Rhodobacter capsulatus because transformational systems have not been shown as efficient methods of introducing DNA to R. capsulatus. For R. capsulatus bacterial conjugation using an Escherichia coli donor can be used to introduce replicating vectors, and non-replicating vectors for targeted chromosomal modifications.
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
The development of this protocol was funded by a grant to J.T.B. from the Canadian Institutes of Health Research.
References
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