Abstract
Olfactory sensory neurons in the main olfactory epithelium (MOE) are responsible for detecting odorants and EOG recording is a reliable approach to analyze the peripheral olfactory function. However, recently we revealed that rodent MOE can also detect the air pressure caused by airflow. The sensation of airflow pressure and odorants may function in synergy to facilitate odorant perception during sniffing. We have reported that the pressure-sensitive response in the MOE can also be assayed by EOG recording. Here we describe procedures for pressure-sensitive as well as odorant-stimulated EOG measurement in the mouse MOE. The major difference between the pressure-sensitive EOG response and the odorant-stimulated response was whether to use pure air puff or use an odorized air puff.
Keywords: Olfaction, Epithelium, EOG, Mouse, Odorants
Materials and Reagents
Equipment
Software
Procedure
A. Preparation of electrodes
B. MOE dissection
C. Configuration of EOG recording
D. EOG measurement
E. Exclusion of artifacts from EOG recording of pressure-sensitive response
Occasionally, artifacts were seen in the EOG recordings due to damaged tissue preparations or other unpredicted reasons. Artifacts could be excluded from pressure-sensitive EOG recording on the basis of following criteria.
F. Data analysis
G.Comparison of pressure-sensitive EOG response with odorant-stimulated EOG response
Recipes
Acknowledgments
The EOG recording method described in this protocol was published in Chen et al. (2013). This research was supported by a National Institutes of Health Grant DC0415 (To Dr Daniel R. Storm).
References
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