Published: Vol 3, Iss 11, Jun 5, 2013 DOI: 10.21769/BioProtoc.779 Views: 11731
Reviewed by: Ru Zhang
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Abstract
Generation of antibodies specific for a protein of interest is a common method in many disciplines. This protocol details the steps in production of a polyclonal antibody in rabbits using a bacterially expressed fusion protein as an antigen. The protocol is generated based on data presented in Wirschell et al.(2013).
Keywords: AntibodyMaterials and Reagents
Equipment
Procedure
Recipes
Note: All buffers can be generated using the concentrated stock solutions provided with the referenced kits and by standard molarity calculations.
References
Article Information
Copyright
© 2013 The Authors; exclusive licensee Bio-protocol LLC.
How to cite
Wirschell, M. and Porter, M. E. (2013). Generation of Polyclonal Specific Antibodies. Bio-protocol 3(11): e779. DOI: 10.21769/BioProtoc.779.
Category
Immunology > Antibody analysis > Antibody-antigen interaction
Biochemistry > Protein > Isolation and purification
Biochemistry > Protein > Immunodetection
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