Abstract
The Infinium Human Methylation 450 BeadChip technology allows the rapid quantitative DNA methylation analysis of more than 485,000 CpG dinucleotides located across the genome. The method utilizes sodium bisulfite treatment of genomic DNA to convert unmethylated cytosine residues into uracils whereas methylated cytosines remain unchanged. Modified DNA is then whole genome amplified, fragmented and hybridized to locus-specific oligomer probes linked to individual beads on a BeadChip. Hybridization is followed by single-base extension of the oligomer with a labeled nucleotide. The BeadChip is subsequently fluorescently stained and scanned to measure the intensities of the beads corresponding to the unmethylated and methylated CpG sites.
Keywords: Epigenetics, DNA methylation, Infinium Methylation Assay, Bisulfite conversion, Cancer Biology
Materials and Reagents
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Acknowledgments
This work was supported by grant awards from Cancer Research UK (C536/A6689), Imperial Experimental Cancer Medicine Centre and Imperial Cancer Research UK Centre.
References
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