Abstract
The number of stomata on leaves is known to be affected by various environmental factors and intrinsic developmental program. Stomatal density and stomatal index are generally used as indicators of the leaf development and the plant growth. This protocol describes an easy, non-destructive method for preparing imprints of the rice leaf surface that is suitable for observation and counting of stomata. Researchers can process many leaf samples at once in the field or in the green house distance from the laboratory.
Keywords: Rice, Stomatal density, CO2, Stomata, Respiration
Materials and Reagents
Equipment
Procedure
Acknowledgments
I am grateful to Ryoko Kaji for her technical assistance. This work was supported by the Kyushu University Interdisciplinary Programs in Education and Projects in Research Development (P&P), a Grant-in-Aid for Scientific Research on Innovative Areas (No. 21114002) and the Ministry of Education, Science and Culture of Japan (No. 22570045).
References
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Sorry for late reply. If your problem still remains unsolved, send your photographs to the following address. I will look for stomata. kkususcb@kyushu-u.org