Abstract
This is a protocol to detect lipid transfer activity of NRF-5, a member of the LPS binding/lipid transfer protein family. The lipid transfer activity is examined by using isotope-labeled cholesterol and liposomes, and tested in two directions (Figure 1): from proteins to liposomes and from liposomes to proteins.
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol is adapted from Zhang et al. (2012) and Infante et al. (2008).
References
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