Abstract
Olfactory ensheathing cells (OECs) can be isolated and purified from a range of postnatal day 7-day to 10-day rat olfactory bulbs. Rat OECs express the CD271/p75NTR receptor and using the “Do-It-Yourself” magnetic nanoparticle EasySep kit from STEMCELL technologies this protocol allows the selective purification of these cells in less than 50 min. Similar procedure can be used for mouse cultures.
Keywords: Olfactory, Glia, Purification, P75NTR, Magnetic beads
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
The protocol was adapted from Franceschini and Barnett, (1996) and Higginson and Barnett, (2011). The work was funded by the MRC.
References
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