Published: Vol 3, Iss 7, Apr 5, 2013 DOI: 10.21769/BioProtoc.531 Views: 14153
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Abstract
The study of fear memory is important for understanding various anxiety disorders in which patients experience persistent recollections of traumatic events. These memories often involve associations of contextual cues with aversive events; consequently, Pavlovian classical conditioning is commonly used to study contextual fear learning. A form of contextual fear conditioning that is becoming increasingly important as an animal model of anxiety disorders uses predator odor as a fearful stimulus. Innate fear responses to predator odors are well characterized and reliable; however, attempts to use these odors as unconditioned stimuli in fear conditioning paradigms have been highly dependent on experimental setup and have produced inconsistent behavioral results. Here we present a contextual fear conditioning paradigm using coyote urine as the unconditioned stimulus, which has been shown to produce consistent contextual freezing in response to fear learning (Wang et al., 2012).
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Acknowledgments
This protocol is adapted from Wang et al. (2012).
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© 2013 The Authors; exclusive licensee Bio-protocol LLC.
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Category
Neuroscience > Behavioral neuroscience > Learning and memory
Neuroscience > Behavioral neuroscience > Animal model
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