In my experiment, I found some different result.
In gel electrophoresis, there is thick band in lower part in agarose gel (Fig 1).
In protocol, there is just a DNA band in upper part...
And also, that lower band is not disappear after MNase treatment (FIg 2).
I could see upper band of 40U was cleaved more than other when I load gel longer (50V, 1hour)as lower band was felt our and disappear (Fig 3).
I have no idea about lower thick band...
Is it normal? or something wrong in my experiment?
Please give me your opinion.
Thank you.
2/1/2016 1:38:12 AM Reply