Abstract
Fusarium root and crown rot is a very important complex disease of small grain cereals worldwide which may lead to very high yield losses. Traditional virulence assays are time consuming and often require plants to be grown in greenhouses or climatic chambers in soil. We describe a rapid laboratory assay for assessing such a disease in wheat and barley seedlings. The method could be successfully used for testing fungal virulence as well as to assess plant resistance.
Materials and Reagents
Equipment
Procedure
Acknowledgments
The present protocol here described in detail was adopted in experiments reported in the following publications: Beccari et al. (2011) and Gardiner et al. (2012). Work was supported by the Australian Grains Research and Development Corporation, an Australian statutory authority.
References
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