Dear all,
I would like to thank you for your protocol. I much appreciated it!
I am a PhD student at Imperial College London.
I hope you don't mind if I ask for some questions.
1. Do you collect samples from healthy donors?
2. Before leukapheresis, do donors receive GCSF?
3. Which initial volume of peripheral blood would be desirable without leukapheresis?
4. Do you think the initial isolation of CD14 and iNKT can be omitted with an acceptable expansion efficiency?
5. How long can you keep culturing and expanding iNKT cells in your experience?
6. Can you see any difference in the expansion efficiency among donors?
Many thanks in advance for your help! I am very grateful indeed!
Best regards,
Antonia Rotolo
4/9/2013 1:08:28 AM Reply