Published: Vol 3, Iss 5, Mar 5, 2013 DOI: 10.21769/BioProtoc.398 Views: 24655
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Abstract
This method is for the immunoprecipitation of Flag-Tagged RNA binding proteins from mammalian cell lines and isolation of the bound RNAs for analysis by quantitative real-time PCR. The RNA binding protein of interest should be tagged with the M2 Flag-tag and expressed in the mammalian cell line of interest (Knuckles et al., 2012). However, specific antibodies for the protein of interest can be used in conjunction with Sepharose G-beads.
Materials and Reagents
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Acknowledgments
This protocol was previously used in Knuckles et al. (2012).
References
Article Information
Copyright
© 2013 The Authors; exclusive licensee Bio-protocol LLC.
How to cite
Vogt, M. and Taylor, V. (2013). Cross-linked RNA Immunoprecipitation. Bio-protocol 3(5): e398. DOI: 10.21769/BioProtoc.398.
Category
Biochemistry > RNA > RNA-protein interaction
Biochemistry > Protein > Immunodetection
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