Abstract
Germinal center is the primary site for B cells to undergo somatic hypermutation and class switching. A recently discovered subset of T cells known as T follicular helper (TFH) cells are essential for germinal center reaction and thus have been the subjects of intensive study in the recent years. This protocol describes a reliable way to stain this population for flow analysis. This protocol was developed or modified in Dr. Anne Davidson’s lab at Feinstein Institute for Medical Research.
Keywords: Flow cytometry, Mouse, Follicular helper T cells, Germinal center
Materials and Reagents
Equipment
Procedure
Gating strategy
Recipes
Acknowledgments
This protocol was developed or modified in Dr. Anne Davidson’s lab at Feinstein Institute for Medical Research, NY, USA. This work was supported by grants from the NY SLE Foundation (RB), Rheuminations, NIH AI082037 and AR 049938-01, NIH (PO1 AI51392 and the Flow Cytometry and Protein Expression and Tetramer Cores of PO1 AI51392).
References
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It is a typo. It was meant to be 200ul. Thank you for your question and we apologize for the error.
Dear Alex: Thank you for your question. Please clarify WHAT info you want regarding the addition of WHICH antibodies as the steps to add antibodies into the samples were described in the protocol with great details. Also I am not exactly sure what you mean by "exclusion of different fluorochromes". Were you asking what fluorochromes cannot be used? Or the compensation of them? Please elaborate on your question, thank you.