Immunoelectron Microscopy in Chlamydomonas Cells   

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Original research article

A brief version of this protocol appeared in:
Biochimica et Biophysica Acta
Aug 2012


The method of immunoelectron microscopy is intended for localization of proteins inside the cells of Chlamydomonas reinhardtii or other microalgae and cyanobacteria. This protocol was used to study localization of carbonic anhydrase Cah3 with antibodies raised in rabbit, though it can be used to localize any other abundant protein. Primary rabbit antibodies are recommended because they react quickly and specifically with proteins of C. reinhardtii. If primary antibodies other than rabbit are used, the blocking procedure and time of incubation with primary and secondary antibodies should be adjusted.

Keywords: Ultrastructure, Chlamydomonas reinhardtii, Carbonic anhydrase, Localization

Copyright: © 2013 The Authors; exclusive licensee Bio-protocol LLC.
How to cite: Sinetova, M. A. and Markelova, A. G. (2013). Immunoelectron Microscopy in Chlamydomonas Cells. Bio-protocol 3(5): e335. DOI: 10.21769/BioProtoc.335.

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