Abstract
This protocol comprises the entire process of immunofluorescence staining on mouse cochlea whole mount, starting from tissue preparation to the mounting of the tissue. This technique provides “three-dimensional” views of the stained components in order to determine the localization of a protein of interest in the tissue in its natural state and environment.
Keywords: Inner ear, Cochlea whole mount, Immunofluorescence, Myosin 7a stain, Vglut3 stain
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
We acknowledge the support of this work by Hearing Research Inc and NIH R21 DC012118-01. We thank Neuron Journal and the Mass Eye and Ear for some illustrating images we used in this paper.
References
If you have any questions/comments about this protocol, you are highly recommended to post here. We will invite the authors of this protocol as well as some of its users to address your questions/comments. To make it easier for them to help you, you are encouraged to post your data including images for the troubleshooting.
If you forgot to rinse your samples from PFA there will be no negative effect to your samples. For your information some protocols use 1% PFA with EDTA when decalcifying.
Thanks for your interest to our protocol.The video was on the Massachusetts Eye and Ear web site but it looks like they removed it.