Abstract
Rice is one of the world’s most important crops, but its production suffers from insect pests. Rice brown planthopper (BPH; Nilaparvata lugens Stål) and striped stem borer (SSB, Chilo suppressalis Walker) are the two most serious pests in rice production. We reported that serotonin is an essential mediator in the interaction between rice and insect. Here, we established a method for extraction and determination of serotonin in rice and BPH.
Keywords: Serotonin, Rice, Artificial diet, BPH, SSB, Insect resistance
Background
Serotonin (5-hydroxytryptamine) is an important signaling molecule in animals. It has also been shown to be distributed widely in plants. In our previous research, we reported that deficiency of serotonin in rice plants enhances its BPH and SSB resistance, and the addition of serotonin in plant growth medium results in a loss of insect resistance in mutants (Lu et al., 2018). Here, we provide detailed protocols for determination of serotonin in rice plants, and in SSB and BPH insects, together with the methods for feeding SSB and BPH in artificial diets (added with exogenous serotonin).
Materials and Reagents
Equipment
Software
Procedure
Data analysis
Statistical analyses were performed using the one-way analysis of variance (ANOVA) programme StaView in these experiments. Data are present in mean values with standard errors as error bars. The differences were considered to be significant when the probability (P) was less than 0.05 in Tukey test.
Notes
All data generated or analyzed during this study are included in this published article and its supplementary information files.
Recipes
Acknowledgments
This study was supported by grants from National Key Research and Development Program of China (2016YFD0102103), Zhejiang Provincial S & T Project on Breeding of Agricultural (Food) Crops (2016C02050-2), China Postdoctoral Research Project (2017M620248).
Competing interests
There are no any conflicts of interest or competing interests.
References
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