Abstract
Proliferative capacity and degranulation are important features of antigen-specific CD8+ T cells. By combining tetramer staining with a CFSE staining, we were able to enumerate the total number of antigen-specific T cells, as well as their number of divisions upon antigen-specific stimulation during a week. In addition, we performed restimulation of these cells, to analyze their ability to secrete cytolytic granules, visualized by CD107a staining.
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol has been adapted from the publication by Hobo et al. (2012). We thank Rob Woestenenk for assistance in flow cytometry and Michel Kester for providing us with tetramers. This work was supported by grants from the Dutch Cancer Society (KWF 2008-4018) and Radboud University Nijmegen (UMCN 2007-34).
References
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