Abstract
Grafting is a very useful technique for testing the transmission of long-distance signals in plants and is used in agriculture and gardening for different purposes. This protocol, based on a previously published one (Jackson et al., 1998), describes a grafting method for potato plants, which has successfully been used to test the transmission of tuber-inducing signals (Martin et al., 2009; González-Schain et al., 2012). We describe first the procedures for plant growth and then the grafting procedure. Although this method has been used for grafting plants that were initially grown in vitro, it should work as well with plants grown from tubers in soil. This protocol probably works well for other plant species, with small modifications.
Keywords: Grafting, Potato, Solanum tuberosum
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol was adapted from Jackson et al. (1998) and Martin et al. (2009) and was optimized in our laboratory by Nahuel D. González-Schain. This work was funded by the Spanish Ministry of Education and Science (grants BIO2002-00933 and BIO2005-00717, co-financed by the European Regional Development Fund) and the Xarxa de Referència en Biotecnologia of the Generalitat de Catalunya. P.S.-L. was supported by the Ramón y Cajal and I3 programs of the Spanish Ministry of Education and Science.
References
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