I was recently alerted that there are some typos and errors in the protocol "Obtaining acute brain slices (https://bio-protocol.org/e2699)" we published last year.

The recipe of: "1- Stock artificial cerebrospinal fluid (ACSF) solution (1 L, store at 4 °C)" has numerical errors and units typos. Please replace it with the following:

Recipes

Stock artificial cerebrospinal fluid (ACSF) solution (1 L, store at 4 °C)
Glucose 10 mM (1.8 g for 1 L)
Potassium chloride 3.2 mM (0.23 g for 1 L)
Sodium chloride 120 mM (7 g for 1 L)
Sodium phosphate monobasic anhydrous 1 mM (0.119 g for 1 L)
Sodium bicarbonate 26 mM (2.18 g for 1 L)
Make up to 1 L with ddH₂O
Verify and adjust pH to 7.3 and osmolarity to 290-300 mOsm.L^-1

References

Papouin, T. and Haydon, P. G. (2018). Obtaining Acute Brain Slices. Bio-protocol 8(2): e2699.