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This is a correction notice.See the corrected protocol

Correction: Obtaining Acute Brain Slices    

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I was recently alerted that there are some typos and errors in the protocol Obtaining acute brain slices we published last year.

The recipe of: "1- Stock artificial cerebrospinal fluid (ACSF) solution (1 L, store at 4 °C)" has numerical errors and units typos. Please replace it with the following:

Recipes

  1. Stock artificial cerebrospinal fluid (ACSF) solution (1 L, store at 4 °C)
    Glucose 10 mM (1.8 g for 1 L)
    Potassium chloride 3.2 mM (0.23 g for 1 L)
    Sodium chloride 120 mM (7 g for 1 L)
    Sodium phosphate monobasic anhydrous 1 mM (0.119 g for 1 L)
    Sodium bicarbonate 26 mM (2.18 g for 1 L)
    Make up to 1 L with ddH2O
    Verify and adjust pH to 7.3 and osmolarity to 290-300 mOsm.L-1

References

  1. Papouin, T. and Haydon, P. G. (2018). Obtaining Acute Brain Slices. Bio-protocol 8(2): e2699.
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Copyright: © 2018 The Authors; exclusive licensee Bio-protocol LLC.
How to cite: Papouin, T. and Haydon, P. G. (2018). Correction: Obtaining Acute Brain Slices. Bio-protocol 8(13): e2945. DOI: 10.21769/BioProtoc.2945.
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