Abstract
This protocol allows to measure the levels of intratumoral hemoglobin from human or rodent fresh samples but also frozen tumors. The advantage of this method is to use very few microliters of biological material for hemoglobin and the protocol is carried out quickly.
Keywords: Cancer, Angiogenesis, Resistance to treatment
Materials and Reagents
Equipment
Procedure
Acknowledgments
Our protocol was adapted from the following article: Grepin et al. (2012). We thank Dr Brahimi-Horn for editorial assistance. Financial support: Contract VEGFIL from the National Institute of Cancer (INCA), the French Association for Cancer Research (ARC), the Foundation de France, the “Conseil Général des Alpes Maritimes”, Roche France and The “Association pour la Recherche sur les Tumeurs du Rein (ARTuR)”.
References
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