Search

A Fluorescent Dye Method Suitable for Visualization of One or More Rat Whiskers   

Download PDF How to cite Favorites Q&A Share your feedback Cited by

In this protocol

Original research article

A brief version of this protocol appeared in:
eLIFE
Jun 2017

Abstract

Visualization and tracking of the facial whiskers is critical to many studies of rodent behavior. High-speed videography is the most robust methodology for characterizing whisker kinematics, but whisker visualization is challenging due to the low contrast of the whisker against its background. Recently, we showed that fluorescent dye(s) can be applied to enhance visualization and tracking of whisker(s) (Rigosa et al., 2017), and this protocol provides additional details on the technique.

Keywords: Whisker, Tracking, Fluorescence, Dye, Tactile perception, Barrel cortex

Background

Over the last 10 years, neuroscientists have begun to focus on sensorimotor processing in behaving rodents. A critical step is to be able to quantify the signals that enter the nervous system from the outside world. For whisker-mediated touch, visualization and tracking of the facial whiskers are required in order to characterize the sensory input. Though many approaches have been employed, only high-speed videography has proven adequate for measuring whisker motion and deformation during interaction with an object. However, whisker visualization and tracking is challenging for multiple reasons, primary among them the low contrast of the whisker against its background. This protocol details a technique for increasing contrast by rendering whiskers fluorescent.

Copyright Rigosa et al. This article is distributed under the terms of the Creative Commons Attribution License (CC BY 4.0).
How to cite:  Readers should cite both the Bio-protocol article and the original research article where this protocol was used:
  1. Rigosa, J., Lucantonio, A., Noselli, G., Fassihi, A., Zorzin, E., Manzino, F., Pulecchi, F. and Diamond, M. E. (2018). A Fluorescent Dye Method Suitable for Visualization of One or More Rat Whiskers. Bio-protocol 8(5): e2749. DOI: 10.21769/BioProtoc.2749.
  2. Rigosa, J., Lucantonio, A., Noselli, G., Fassihi, A., Zorzin, E., Manzino, F., Pulecchi, F. and Diamond, M. E. (2017). Dye-enhanced visualization of rat whiskers for behavioral studies. Elife 6.
Q&A

Please login to post your questions/comments. Your questions will be directed to the authors of the protocol. The authors will be requested to answer your questions at their earliest convenience. Once your questions are answered, you will be informed using the email address that you register with bio-protocol.
You are highly recommended to post your data including images for the troubleshooting.

You are highly recommended to post your data (images or even videos) for the troubleshooting. For uploading videos, you may need a Google account because Bio-protocol uses YouTube to host videos.