Abstract
This protocol is used to compare the in vivo redox status of Escherichia coli and Vibrio cholerae protein before and after HOCl treatment. For example, I examined whether the EF-Tu protein is reduced or oxidized in the referenced publication. This protocol should work for other proteins and other oxidative stress treatments. You will need the antibody for your protein to visualize your protein on western blot.
Keywords: Thiol trap, Oxidation, Redox status
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
The protocol described here is adapted from one reported previously (Wholey et al., 2012).
References
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