Abstract
This system is one of a chronic allergic inflammation model. A single subcutaneous injection of antigens elicits a delayed-type response manifested by ear swelling. The entire response consists of three phases: Immediate, late, and chronic. The early-phase swelling peak within 1 h after allergen challenge is followed by the late-phase swelling 6-10 h late. Importantly, the ear swelling at the late-phase started on day2 and peak on day4. This late-phase ear swelling response is defined as IgE-mediated chronic allergic inflammation (IgE-CAI) and mainly regulated by basophils.
Materials and Reagents
Equipment
Procedure
Acknowledgments
This protocol was developed and implemented by Dr. Masato Kubo at Division of Molecular Pathology, Tokyo University of Science, Chiba, Japan and Dr. Hiromasa Inoue at Department of Pulmonary Medicine, Kagoshima University, Kagoshima, Japan. This work was supported by a Grant-in-Aid-of-Scientific Research in Priority Areas of the Ministry of Education, Culture, Sports, Science, and Technology of Japan and the Program for Promotion of Fundamental Studies in Health Sciences of the National Institute of Biomedical Innovation.
References
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