Abstract
A lot of transcriptional profiling in plant and animals has used RNAs samples from many different cell types. The laser-capture microdissection (LCM) can identify and harvest pure cellular populations directly from heterogenous tissues based on histological identification. The molecules or protein isolated from LCM-captured cells can be suitable for single cell type analysis by using chip expression profiling or sequencing.
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol is adapted from Kerk et al. (2003) and Jiao et al. (2009).
References
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