Abstract
This is an accurate method to assess the rate of chlorophyll biosynthesis in vivo in cyanobacteria. Given that labeled glutamate is used as the very early precursor of chlorophyll together with a short pulse of labeling (30 min), this method provides information about the metabolic flow through the whole chlorophyll biosynthetic pathway on a short timescale.
Keywords: Chlorophyll biosynthesis, 14C radiolabeling, Cyanobacteria, Synechocystis, Thin Layer Chromatography
Materials and Reagents
Equipment
Procedure
Acknowledgments
We acknowledge the financial support from the project Algatech (CZ.1.05/2.1.00/03.0110) and Czech Science Foundation, project no. P506/12/1522.
References
If you have any questions/comments about this protocol, you are highly recommended to post here. We will invite the authors of this protocol as well as some of its users to address your questions/comments. To make it easier for them to help you, you are encouraged to post your data including images for the troubleshooting.